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. 2013 Oct 11;8(10):e76667. doi: 10.1371/journal.pone.0076667

Figure 4. Effect of depletion or inactivation of PAR-2 on Erk1/2 phosphorylation and cell proliferation.

Figure 4

(A) Western blot analysis using anti-PAR-2 antibody N-19 showed that the siRNA against PAR-2 caused a significant decrease (55%) in PAR-2 expression in BART cells compared with scrambled treatment. (B) Flow cytometry analysis further confirmed that the knockdown of PAR-2 led to a 60% reduction in PAR-2 surface expression in comparing with scrambled control cells. (C) siRNA against PAR-2 abrogated trypsin-induced Erk1/2 phosphorylation in BART cells. (D) Showing that siRNA knockdown PAR-2 inhibited trypsin-induced cell proliferation in both BART and OE33 cells. Ctrl: non-transfection; Scmbl: scramble control; siPAR2: siRNA PAR-2 knockdown. (E) Inhibition of PAR-2 activity by PAR-2 antagonist ENMD or anti-PAR-2 antibody SAM-11 abolished trypsin-induced proliferation.