Table 2. List of strains and plasmids used in this study.

Strain or plasmid	Description	Reference
T. turnerae strains
T7901	Wild-type	Distel et al., 2002 [21]
TtAH03	tnbF::Cmr	This study
E. coli strains
TOP10	F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG	Invitrogen
S17–1 λpir	thi pro hsdR hsdM+ recA RP4–2-Tc::Mu-Km::Tn7λpir	Simon et al., 1983 [65]
Plasmids
pCR2.1	T-vector, Kmr, Ampr	Invitrogen
pDM4	Suicide vector, sacB gene, R6K origin, Cmr	Milton et al., 1996 [66]
pDMtnbF	Portion of tnbF gene cloned into pDM4	This study