Table 1. Primers used for qRT-PCR.

Forward (Fwd) and reverse (Rev) primers used to determine changes in gene expression by qRT-PCR. GenBank accession numbers for the transcript variants recognized by primer pairs are indicated.

Gene	Strand	Primer Sequence	GenBank Accession Number
Acan	Fwd	5′-ATGGTCCTTCTATGACATACACTCCCCG-3′	NM_007424
	Rev	5′-TTGTTACAGCGCCACCAAGG-3′
Adamts4	Fwd	5′-GATGTGTGCAAGCTTACCT-3′	NM_172845
	Rev	5′-CATCCGTAACCTTTGGAGA-3′
Col2a1	Fwd	5′-GACCCAAACACTTTCCAACCGCAGT-3′	NM_031163; NM_003396
	Rev	5′-TCATCAGGTCAGGTCAGCCATT-3′
Col10a1	Fwd	5′-CAGGCTTTCTGGGATGCCGCTTGT-3′	NM_009925
	Rev	5′-GGGCACCTACTGCTGGGTAA-3′
Hes1	Fwd	5′-ACCAAAGACGGCCTCTGAGCACAGAAAGT-3′	NM_008235
	Rev	5′-ATTCTTGCCCTTCGCCTCTT-3′
Hey1	Fwd	5′-ATCTCAACAACTACGCATCCCAGC-3′	NM_010423
	Rev	5′-GTGTGGGTGATGTCCGAAGG-3′
Hey2	Fwd	5′-AGCGAGAACAATTACCCTGGGCAC-3′	NM_013904
	Rev	5′-GAGGTAGTTGTCGGTGAATTGG-3′
HeyL	Fwd	5′-CAGTAGCCTTTCTGAATTGCGAC-3′	NM_013905
	Rev	5′-GCTTGGAGGAGCCCTGTTTCT-3′
Il6	Fwd	5′-CGGCCTTCCCTACTTCACAAGTCCG-3′	NM_031168
	Rev	5′-CAGGTCTGTTGGGAGTGGTATCC-3′
Mmp13	Fwd	5′-GGAAGACCTTGTGTTTGCAGAGC-3′	NM_008607
	Rev	5′-CACTGTAGACTTCTTCAGGATTCCCG-3′
Prg4	Fwd	5′-CGCCTTTTCCAAAGATCAATACTA-3′	NM_021400; NM_001110146
	Rev	5′-GTGGTAATTGCTCTTGCTGTT-3′
Rpl38	Fwd	5′-AGAACAAGGATAATGTGAAGTTCAAGGTTC-3′	NM_001048057; NM_001048058; NM_023372
	Rev	5′-CTGCTTCAGCTTCTCTGCCTTT-3′
Sox9	Fwd	5′-CCTACTACAGTCACGCAGCCG-3′	NM_011448
	Rev	5′-GGGTTCATGTAAGTGAAGGTGGA-3′