Figure 3. Activated Stat92E induces hinge-specific genes and represses notum genes.

(A) In a wild type mid- to late third instar disc, Stat92E activity (green) largely overlaps with that of Msh (red) in the proximal hinge. Wg is blue. A′ is single channel for Msh; A″ for 10xSTAT-GFP.

(B) Msh (red) is lost in a cell-autonomous manner in Stat92E clones (B′, arrow), which lacks GFP. Clone boundaries are outlined by dashed yellow lines. B′ is single channel for Msh; B″ for GFP.

(C) Zfh2 (magenta) is reduced in a cell-autonomous manner (C′, arrows) in Stat92E clones, which lack GFP. C′ is single channel for Zfh2.

(D) Zfh2 (magenta) is ectopically induced in a cell-autonomous manner within some regions of Hop-expressing clones, for example at the edges of clones residing in the notum (D′, arrows). D′ is single channel for Zfh2.

(E) ds-lacZ (magenta) is reduced in a cell-autonomous manner (E′, arrows) in Stat92E clones, which lack GFP. E′ is single channel for ds-lacZ; E″ for GFP.

(F) Msh (red) can be induced in a cell autonomous manner (F′, arrow) in Hop-expressing clones residing in the notum. Dlg is blue. F′ is a close-up of the Hop flip-out clone. F″ is the single for Msh.

(G) Msh (green) and Ara (red) have mutually exclusive expression patterns. Dlg is blue.

(H) In a Stat92E clone, Ara (red) expression is expanded into the into the lateral hinge (H′, red arrow). By contrast, Ara is not increased in neighboring heterozygous cells that contain one functional copy of Stat92E (H′, green arrow). H′ is single channel for Ara.

(I) In Hop-expressing clones (green), Ara (red) is repressed in a cell-autonomous manner (arrows in I′). Msh is blue. Most often Ara repression is associated with upregulation of Msh within the Hop flip-out clone (I″, I″, green arrows). However, less frequently, we observe repression of Ara in the Hop flip-out clone without increases in Msh (I′, I″, red arrows). I′ is single channel for Ara; I″ for Msh.