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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Dev Biol. 2013 Aug 23;382(2):413–426. doi: 10.1016/j.ydbio.2013.08.016

Figure 5. Wg signaling is dominant to JAK/STAT signaling in the wing hinge.

Figure 5

(A) TCFDN-expressing clones, which inhibit Wg signaling, (marked by βGal (red)) exhibit cell-autonomous loss of 10xSTAT-GFP (green) and the hinge epithelial fold (B′, B″, arrows). Wg is blue. B′ is a close of the area outlined in yellow in B. B″ is single channel for 10xSTAT-GFP.

(B) The wgspd-fg-LacZ reporter (magenta), corresponding to wg expression in the Wg IR (and at the dorso-ventral boundary), shifts proximally in Stat92E clones, which lack GFP. Red and green arrows in C′, C″ indicate in proximal movement of the wgspd-fg-LacZ reporter within Stat92E clone boundaries.

(C, D) 10xSTAT-GFP (green) activity is reduced in a third instar wgspd-fg mutant disc, particularly the distal most fold of reporter expression (C′, D′, arrow). The Wg IR (red) is also lost in this mutant background (C″ D″). C′, D′ is single channel for 10xSTAT-GFP; C″, D″ for Wg.

(E–L) In a second instar (E) and third instar (G) disc from +/SM5~TM6B disc (labeled WT), 10xSTAT-GFP (green) is expressed in two folds in the hinge, one proximal and one distal. Nub is Red and Tsh is blue. F, H show xz scans through the region indicated by the white lines in E and G. Stat92E activity (F″, H″) is concentrated in two domain between the Nub (F″, H″) and Tsh (F‴, H‴) domains. In a second instar wgspd-fg mutant disc, Stat92E activity (green) (I, I′) appears similar to sibling controls (E, E′). However, by mid-third instar, the distal domain of 10xSTAT-GFP expression is greatly reduced in wgspd-fg (K, K′) compared to sibling controls (G, G′). J, L show xz scans through the region indicated by the white lines in I and K. In a second instar wgspd-fg disc, Stat92E activity (J″) is concentrated in two domain between the Nub (F″) and Tsh (F‴) domains similar to sibling controls (F″). However, in a third instar wgspd-fg disc, Stat92E activity (L″) is greatly reduced in the distal fold (L″, arrow), in stark contrast to a sibling control (H″). F, H, J, L is the merge where 10xSTAT-GFP is green, Nub is red and Tsh is blue. F′, H′, J′, L′ are single channels for 10xSTAT-GFP; F″, H″, J″, L″ for Nub and F‴, H‴, J‴, L‴ for Tsh.