Fig. 10.
Developmental appearance of immunolabelling for Cx36 and vglut-1 in SpVN of rat at postnatal day 15. (A) Images of the same field labelled for Cx36 (red) and vglut-1 (green), with Nissl fluorescence counterstain (blue). Labelling for Cx36 (A1, arrows) and vglut-1 (A2, arrows) is dense on neuronal somata and moderate on initial dendritic segments. Cx36-puncta appear as clusters of puncta co-localized with vglut-1 on neuronal somata (A3, arrows) or as non-colocalized puncta, and dispersed Cx36-puncta not associated with Nissl-stained neurons are readily evident.