Figure 1. RSV attenuates the development of 4T1 breast cancer and lung metastasis.

Female BALB/c mice were i.p. injected with either 20 μg RSV or 50 μg RSV every other day starting from day 3 post s.c. challenge with 4T1 cancer cells (5×10⁴). Control tumor-bearing mice (mock) were injected with empty vehicle. Y-axis in A shows tumor size ² ± SEM measured at indicated times (X-axis) and in B depicts number of lung metastatic foci ± SEM at day 28 post tumor challenge in 4–5 mice per group experiment reproduced three times. The proportion (%) of tBregs (CD81^HiCD25⁺ gated on CD19⁺ cells in spleen (C) and lymph node (D), respectively) and splenic Tregs (Foxp3⁺ gated on CD4⁺ cells, E) was measured in mice shown in A and B. Dot blots in C–E are representative data of the experiments shown in lower panels (C–E). From hereon, NS: statistically no significant; *p<0.05; ** p<0.01; ***p<0.001.