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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 21;0:384–394. doi: 10.1016/j.freeradbiomed.2013.04.011

Figure 5.

Figure 5

Transient and localized changes in cellular ROS levels in response to 8-oxoG exposure. Cells were transfected with pHyPer-Cyto, pHyPer-dMito or pHyPer-Nuc and 72h later challenged with 8-oxoG (10 μM) or H2O2 (10 μM). At times indicated, cells were washed in PBS, fixed with formalin (3.7%), dryed and mounted on microscope slides. Images were taken by a NIKON Eclipse Ti System (magnification x125). (A), H2O2 exposure-induced changes in fluorescence in sub-cellular compartments of cells. (B), Localized ROS generation by 8-oxoG exposure of cells. Scale bars: 20 μm.