Table 1.
Effect of culture conditions for production of extracellular protease from B. licheniformis P003 in shake-flask cultivation
| Culture condition | Protease activity (U/ml) | Relative activity (%) | Protease activity (U/mg) | Total soluble protein (mg/ml) |
|---|---|---|---|---|
| Mean±SE | Mean±SE | Mean±SE | ||
| Initial pH | ||||
| 8.0 | 39.01±0.190 | 43.68 | 43.72±1.22 | 0.889±0.021 |
| 8.5 | 81.28±0.199 | 91.02 | 86.13±2.12 | 0.944±0.021 |
| 9.0 | 89.30±0.661 | 100.00 | 104.10±1.53 | 0.858±0.016 |
| 9.5 | 79.38±0.143 | 88.89 | 83.81±1.51 | 0.947±0.016 |
| 10.0 | 80.55±0.194 | 90..20 | 94.41±1.45 | 0.853±0.015 |
| 10.5 | 74.62±0.562 | 83.56 | 90.70±5.48 | 0.825±0.057 |
| 11.0 | 10.91±0.185 | 12.22 | 14.74±0.09 | 0.74±0.010 |
| Incubation temperature (°C) | ||||
| 32 | 39.08±0.096 | 72.21 | 35.33±0.11 | 1.106±0.006 |
| 35 | 45.10±0.070 | 83.33 | 40.60±0.40 | 1.112±0.013 |
| 37 | 48.71±0.026 | 90.00 | 42.42±0.22 | 1.143±0.014 |
| 40 | 50.19±0.053 | 92.74 | 43.16±0.49 | 1.155±0.023 |
| 42 | 54.12±0.056 | 100.00 | 43.10±0.27 | 1.255±0.007 |
| 45 | 43.90±0.098 | 81.12 | 35.17±0.20 | 1.247±0.010 |
| Incubation period (hr) | ||||
| 24 | 9.62±0.062 | 20.00 | 7.80±0.07 | 1.231±0.008 |
| 48 | 48.10±0.062 | 100.00 | 40.74±0.17 | 1.178±0.008 |
| 72 | 36.68±0.046 | 76.26 | 30.51±0.12 | 1.200±0.007 |
| 96 | 34.27±0.072 | 71.25 | 29.92±0.11 | 1.143±0.007 |
| 120 | 22.85±0.046 | 47.51 | 20.00±0.04 | 1.141±0.005 |
| Inoculums volume (%, ml) | ||||
| 0.5 | 13.89±0.026 | 25.95 | 16.31±0.09 | 0.849±0.008 |
| 1.0 | 35.48±0.026 | 66.29 | 41.51±0.20 | 0.853±0.006 |
| 1.5 | 36.68±0.053 | 68.54 | 43.12±0.17 | 0.848±0.008 |
| 2.0 | 53.52±0.030 | 100.00 | 58.54±0.37 | 0.915±0.006 |
| 2.5 | 19.24±0.036 | 35.95 | 27.15±0.38 | 0.709±0.009 |
| 3.0 | 13.23±0.026 | 24.72 | 19.23±0.17 | 0.689±0.008 |
| 3.5 | 12.38±0.035 | 53.03 | 23.31±0.39 | 0.531±0.010 |
Data represent as mean ± standard error (SE) for three replicates.