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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Exp Eye Res. 2013 Jul 18;0:263–273. doi: 10.1016/j.exer.2013.06.026

Figure 4.

Figure 4

(A–I) Detection of LC3 in mouse lenses by immunofluorescence analysis. (A–C) Anterior region: (A) Wild type lens; (B) αB-R120G heterozygous mutant lens; (C) αB-R120G homozygous mutant lens. (D–F) Posterior region: (D) Wild type lens; (E) αB-R120G heterozygous mutant lens; (F) αB-R120G homozygous mutant lens. (G–I) Equatorial region: (G) Wild type lens; (H) αB-R120G heterozygous mutant lens; (I) αB-R120G homozygous mutant lens. Red, LC3; Blue, DRAQ 5. c, lens capsule; e, lens epithelium; f, lens fibers. The intensity of the blue fluorescence has been enhanced in these images to clearly visualize the lens capsule. Scale bar, 60 µm. (J) Immunoblot analysis of LC3-1, LC3-II and F-actin in mouse lenses. The molecular weight marker proteins are in the left lane, and the size of the markers is indicated. The bold arrow on the right indicates the F-actin band (red). The lower two arrows indicate the LC3-I and LC3-II bands, respectively. WT, Wild type lens; HET, αB-R120G heterozygous mutant lens; HOMO, αB-R120G homozygous mutant lens.