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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Oncogene. 2013 Jan 14;33(2):10.1038/onc.2012.563. doi: 10.1038/onc.2012.563

Figure 3.

Figure 3

sEcad facilitates SCC cell invasion via MMP-2 and MMP-9. (a) Serum-starved PAM212 and (b) CC4A cell invasion through Matrigel in response to rmEcad/Fc administration. Insert: Representative 0.5% crystal violet staining of rmEcad/Fc treated PAM212 or CC4A cells invading through Matrigel. Scale bars, 250 μm. (c) MMP levels and activities in the presence or absence of rmEcad/Fc were quantified by ELISA and gelatin zymography, respectively. (d) Invasive potential of PAM212 cells treated with rmEcad/Fc was determined after pretreatment with 20μM GM6001 for 1 h, and invasion though Matrigel analyzed in the presence of rmEcad/Fc (20μg/mL) and 10μM GM6001 for an additional 22h. All error bars, ± SEM. All quantitative data were generated from a minimum of three replicates.