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. Author manuscript; available in PMC: 2014 May 14.

Published in final edited form as: Biochemistry. 2013 May 1;52(19):10.1021/bi400018h. doi: 10.1021/bi400018h

(a) Circular dichroism spectra of dark-adapted wtPYP (−) and cPYP () (pH 7.5 5 mM sodium phosphate buffer, 20°C) (b) Temperature denaturation titrations of dark-adapted wtPYP (−) and cPYP () (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl) (c) Guanidinium refolding curve for dark-adapted wtPYP (−) and cPYP (). (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl, 20°C).

Inline graphic — (a) Circular dichroism spectra of dark-adapted wtPYP (−) and cPYP () (pH 7.5 5 mM sodium phosphate buffer, 20°C) (b) Temperature denaturation titrations of dark-adapted wtPYP (−) and cPYP () (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl) (c) Guanidinium refolding curve for dark-adapted wtPYP (−) and cPYP (). (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl, 20°C).