Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 May 14.

Published in final edited form as: Biochemistry. 2013 May 1;52(19):10.1021/bi400018h. doi: 10.1021/bi400018h

(a) Recovery of dark-adapted wtPYP (−) and cPYP () after irradiation with 450 nm light (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl, 20°C) (b) pH dependence of dark-state recovery of cPYP as a function of pH (100 mM NaCl, 20°C). The fitted curve is given by: k_obs = k_max /(1 + 10^{(pK₁ − pH)} + 10^{(pH − pK₂)}) with pK₁ = 6.3 ± 0.1 and pK₂ = 9.3 ± 0.1

Inline graphic — (a) Recovery of dark-adapted wtPYP (−) and cPYP () after irradiation with 450 nm light (pH 7.5 Tris-acetate EDTA buffer, 100 mM NaCl, 20°C) (b) pH dependence of dark-state recovery of cPYP as a function of pH (100 mM NaCl, 20°C). The fitted curve is given by: k_obs = k_max /(1 + 10^{(pK₁ − pH)} + 10^{(pH − pK₂)}) with pK₁ = 6.3 ± 0.1 and pK₂ = 9.3 ± 0.1