Fig. 1. Dose response curves of IP production by WT and mutant GnRH receptors in response to Buserelin.
IP production was assessed in response to the indicated doses of Buserelin. A) COS-7 cells were transiently transfected with 25 ng of human WT GnRH receptor or mutant receptor plus 75 ng of empty vector (closed symbols) or human calnexin (open symbols). B) COS-7 cells were transiently transfected with 25 ng of rat WT GnRH receptor or mutant receptor plus 75 ng of empty vector (closed symbols) or rat calnexin (open symbols) as described in Materials and Methods. Means ± S.E.M.s are shown for three independent experiments, each performed in replicates of four.