Fig. 4. Binding analysis using 125I-Buserelin in COS-7 cells transfected with human and rat GnRHR and calnexin.
The cells were transfected with 25 ng of human and rat WT or mutant receptors plus 75 ng of empty vector (solid bar) or human and rat calnexin (gray bar). Means ± S.E.M.s are shown for three independent experiments, each performed in replicates of four. *p < 0.05 vs same groups with calnexin and all groups no calnexin.