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EC50 and maximal Buserelin stimulated inositol phosphate production of the WT and mutants rat GnRHRs (mean ±SEM from 3 independents experiments).
| EC 50 (pM) | Maximal IP production (cpm/500μl) | |
|---|---|---|
| rWT | 84 ± 18 | 4095 ± 252 |
| rWT+calnexin | 92 ± 18 | 3066 ± 264 |
| rWT+K191 | 97 ± 16 | 3531 ± 172 |
| rWT+K191+calnexin | 117 ± 17 | 3191 ± 172 |
| rWT+K191+human motif | 404 ± 11 | 1571 ± 58 |
| rWT+K191+human motif+calnexin | 500 ± 60 | 880 ± 17 |
| rWT+human motif | 351 ± 86 | 2274 ± 65 |
| rWT+human motif+calnexin | 511 ± 15 | 1617 ± 85 |
