Skip to main content
. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: ACS Chem Biol. 2013 May 13;8(7):1567–1575. doi: 10.1021/cb400170b

Figure 1. Method design.

Figure 1

(A) Central steps of sample preparation for mass spectrometric quantification of R-Ado. (B) PAR can be quantitatively detached from acceptor proteins by alkaline treatment. Subsequent digestion of PAR with phosphodiesterase and alkaline phosphatase releases adenosine from the PAR termini and other unique nucleosides, i.e., ribosyladenosine (R-Ado) and diribosyladenosine (R2-Ado), which are characteristic for the linear or branched part of the polymer, respectively. (C) LC-MS/MS chromatogram of Ado, R-Ado and R2-Ado obtained from digested PAR synthesized in vitro.