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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: ACS Chem Biol. 2013 May 13;8(7):1567–1575. doi: 10.1021/cb400170b

Figure 4. DNA-damage induced dynamics of cellular PARylation.

Figure 4

(A) Dose response curves of PAR levels in COPF5 cells and PBMCs (isolated from the same donor, inter-day variability) treated with H2O2 for 5 min. Curves were fitted using a sigmoidal model with variable slope. (B) Time course of PAR formation in COPF5 cells and PBMCs treated with 100 μM H2O2. Data represent means ± SEM from at least three independent experiments. In each experiment PBMCs were isolated from one donor on different days. (C) Assessment of PAR branching by evaluation of the R2-Ado/R-Ado area ratios in COPF5 cells and PBMCs upon H2O2 stimulation as indicated.