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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 11;191(8):4348–4357. doi: 10.4049/jimmunol.1103621

Figure 1. Increased expression of IL-17 by Th17 cells regulates entry of neutrophils to TDLNs following induction of sterile inflammation by PDT.

Figure 1

(A) BALB/c Colo26-HA tumor-bearing mice were subjected to PDT. TDLNs were harvested at the indicated time points post PDT to measure IL-17 mRNA by quantitative RT-PCR. The amount of IL-17 message was normalized to GAPDH and is reported as IL-17 mRNA copies. Each group contains a total of 6 mice and the experiment was repeated once. (B) BALB/c Colo26-HA tumor-bearing mice were subjected to PDT. TDLNs were harvested at the indicated time points post PDT and a single-cell suspension was generated. The absolute number of Th17 in the TDLN was analyzed by flow cytometry and is defined by CD3+CD4+RORγt+iIL-17+. Each group contains a total of 6 mice and the experiment was repeated once. (C) Il17ra−/− or BALB/c mice bearing Colo26-HA tumors were subjected to PDT. (D) BALB/c mice bearing Colo26-HA tumors were treated with 100 μg anti-IL-17A antibodies or isotype control intravenously immediately before PDT. (B – C) TDLNs were harvested at the indicated time points and a single-cell suspension was generated. The absolute number of neutrophils in the TDLN was analyzed by flow cytometry and is defined by CD11b+Ly-6GhiLy-6C+F4/80−CD11c−. Each group contains a total of at least 9 mice and the experiment was repeated twice. Error bars represent mean ± SEM. *P<0.05 to 0h; #P<0.05 to BALB/c.