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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 11;191(8):4348–4357. doi: 10.4049/jimmunol.1103621

Figure 4. Neutrophils gain entry to TDLNs following induction of sterile inflammation through HEV interactions.

Figure 4

TDLNs were harvested at the indicated time points post PDT. A single-cell suspension was generated and analyzed by flow cytometry for the number of neutrophils per TDLN in the presence of (A) 100 μg isotype control or 100 μg anti-L-selectin antibodies or (B) 100 μg isotype control or 100 μg anti-PNAd antibodies. (C) Immunofluorescence staining of TDLNs for PNAd and ICAM-1 was performed as described in Methods. The numerical value represents the mean fluorescence intensity (MFI). Each group contains a minimum of 9 mice and the experiment was repeated twice. Error bars represent mean ± SEM. *P<0.05 to 0h; #P<0.007 to Isotype.