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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 11;191(8):4348–4357. doi: 10.4049/jimmunol.1103621

Figure 6. IL-17 regulates induction of CXCL2 in TDLNs post PDT.

Figure 6

Il17ra−/− or BALB/c Colo26-HA tumor-bearing mice were subjected to PDT. TDLNs were harvested at the indicated time points post PDT to measure expression of CXCL2 mRNA or protein. (A) Quantitative RTPCR was performed and the amount of CXCL2 message was normalized to GAPDH and is reported as CXCL2 mRNA copies. (B) An ELISA kit (R&D Systems) specific for murine CXCL2 was used and the amount of CXCL2 protein per μg total protein is reported. Each group contains a minimum of 9 mice and the experiment was repeated twice. Error bars represent mean ± SEM. *P<0.05 to 0h; #P<0.05 to BALB/c.