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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 11;191(8):4348–4357. doi: 10.4049/jimmunol.1103621

Figure 7. Regulation of IL-1β by IL-17RA enhances CXCL2 expression and neutrophil access to TDLNs post induction of sterile inflammation.

Figure 7

(A) Il17ra−/− or BALB/c Colo26-HA tumor-bearing mice were subjected to PDT. TDLNs were harvested at the indicated time points post PDT and IL-1β protein expression was measured by ELISA (R&D Systems). The amount of IL-1β protein per μg total protein is reported. (B) Colo26-HA tumor-bearing BALB/c mice were treated with 100 μg isotype control or 100 μg anti-IL-1β antibodies intravenously immediately prior to PDT. TDLNs were harvested at the indicated time points to measure CXCL2 protein expression by ELISA (R&D Systems). The amount of CXCL2 protein per μg total protein is reported. (C) Colo26-HA tumor-bearing BALB/c mice were treated with 100 μg isotype control or 100 μg anti-IL-17A or 100 μg anti-IL-1β antibodies either alone or in combination intravenously immediately prior to PDT. TDLNs were harvested at the indicated time points post PDT and a single-cell suspension was generated. The number of neutrophils was determined by flow cytometry. Each group contains a minimum of 9 mice and the experiment was repeated twice. Error bars represent mean ± SEM. *P<0.04 to 0h; #P<0.04 to Isotype or BALB/c.