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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: J Control Release. 2013 Jun 28;171(3):288–295. doi: 10.1016/j.jconrel.2013.06.023

Fig. 5. Thrombin cleavage of GST-GFP from with PEGDA microspheres.

Fig. 5

(A) Release of GFP from GST-GFP PEGD-GSH microspheres in the presence of (Inline graphic) 0, 11 (Inline graphic), 22 (Inline graphic), 44 (Inline graphic), or 110 (Inline graphic) nM thrombin as detected by fluorescent GFP signal. Each point represents the mean plus or minus the standard deviation of three independent samples. (B) Representative brightfield and epifluorescent micrographs depicting GST-GFP (green) associated with microspheres before (top row) or after (bottom row) release in response to thrombin (1.8 µM). (C) Rate (v) of GFP liberation from GST-GFP (unbound; Inline graphic) and GST-GFP (bound; Inline graphic) bound to PEGDA-GSH microspheres for the linear portion of the rate curve, typically the first 1 hour. Each point represents the mean plus or minus the standard deviation of three independent samples.