Table 2.
Parameter | Calculated Value | Observed Value | |
---|---|---|---|
Particle | Diameter (µm) | - | 46±16 |
Surface Area (µm2) | - | 6,647±804 | |
Mesh Size (nm) | - | 4.6±0.2 | |
GST-GFP | Cross Sectional Area† (µm2/molecule) | 1.4×10−5 | - |
Capacity§‡ (molecule/microsphere) | 4.7×108 | 9.9±2.5×108 |
Estimated based upon the crystal structure of a GST dimer and predicted structure of the fusion proteins.
Estimated from the observed microsphere diameter and estimated cross sectional area of GST-GFP.
Observed value was determined from thrombin release from microspheres.