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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 13;191(8):4423–4430. doi: 10.4049/jimmunol.1300042

Figure 5. Analysis of cellular dynamics in BAL and draining MLN of tolerized mice following mucosal sensitization.

Figure 5

A) Total BAL cell recovery 18hr after each of three sensitizations with either LPSlo/OVA or LPShi/OVA. B–E) Flow cytometric analysis of BAL (B, D) and MLN (C, E) cells after sensitization. Absolute numbers of CD3+ CD4+ Foxp3+ cells (B–C) and CD3+ CD4+ CD44hi Foxp3- cells (D–E) are reported. F–G) Recovery of CD3+ CD4+ Foxp3+ and Foxp3- subsets were determined by flow cytometry. The ratio of CD4+ non-Treg to CD4+ Treg cells in BAL (F) and MLN (G) was determined by dividing the number of non-Treg by the number of Treg cells. n = 6–15 per group, * = p<0.05.