A, Micrographs are of representative primary SCCs grouped by invasion pattern and their corresponding PDXs. Dual label IHC of PDXs for E-cadherin (brown) and vimentin (red) is shown together with digitally pseudo-colored images, in which E-cadherin is green, vimentin is red, and hematoxylin is blue. 20x. B, Vimentin positive area is compared between groups with high and low risk invasion patterns, quantitated as a percentage of total (E-cadherin+vimentin) staining area. Areas are defined as the mean ± SEM of three 40x fields. P<1×10-6 between groups. C, IF costaining of vimentin (red) and zeb-1 (green) is shown in sections of PDXs with high risk invasion patterns. 40x. Bars=100μm.