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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Mol Biol. 2013 Jul 16;425(21):3919–3936. doi: 10.1016/j.jmb.2013.07.014

Figure 1.

Figure 1

The change in the folding free energy (ΔΔG) may be evaluated using two different methods: (1) using the difference in the folding free energy values calculated from the transition from an unfolded to a folded state of the wild type (ΔGWT(folding)) minus the mutant type (ΔGMT(folding)) shown with black arrows; or (2) using the difference in the folding free energy between the folded state of the wild type and mutant type (ΔGfolded(WT-MT)) minus the folding free energy between the unfolded state of the wild type and mutant type (ΔGunfolded(WT-MT)) shown with red arrows.