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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Biochemistry. 2013 May 16;52(21):10.1021/bi301662w. doi: 10.1021/bi301662w

Figure 8.

Figure 8

Nano-LC-LTQ Orbitrap identification of the αA66-80 peptide interaction with C-terminal extension of αB-crystallin. (A) Extracted ion chromatogram of tryptic digest of cross-linked complex. Signal of doubly charged peptides with m/z 806.91 and 808.93, cross-linked with light (d0) and heavy (d4) precursor ions presenting a mass difference of 2.02 Da is indicated by arrow. (B) Fragmentation mass spectrum (both CID and HCD) of the identified precursor ion (m/z 806.9) obtained using MS/MS. The identified y and b ions are indicated. (C) The interacting region of the N-terminal amino group of 66th residue of αA66-80 with Lys166 of αB-crystallin is shown in the identified cross-linked sequences.