Table 2.
Identification of potential αB-crystallin and αA66-80 crosslink pairs from the LC-MS data using GPMAWa,b
| Observed mass pairs[MH+] | Intensity | Actual mass[MH+] | αB- crystallin sequence | αA66-80 sequence | type | Delta | ppm |
|---|---|---|---|---|---|---|---|
|
1612.820 1616.843 |
161427 189204 |
1612.818 | 164–174 | 1–3 | X-link | −0.002 | −1 |
| 1979.082 1983.106 |
484245 703659 |
1979.085 | 70–74 | 4–13 | X-link | 0.003 | 1 |
| 2361.165 2365.192 |
1337170 1336468 |
2361.172 | 75–90 | 1–3 | X-link | 0.007 | 3 |
| 2189.084 2193.114 |
206855 229689 |
2189.095 | 93–107 | 1–3 | X-link | 0.011 | 5 |
|
1865.009 1869.034 |
342231 596374 |
1865.013 | 91–103 | 1–3 | X-link | 0.004 | 2 |
a
The deconvoluted peak lists from MS-spectra were compared with theoretically generated cross-linked peptide masses. The theoretical peak lists were generated using GPMAW. The table gives the observed mass in the LC-MS, actual mass (theoretical) generated by GPMAW, the amino acid segments in the interacting partners. Delta corresponds to mass deviation between the observed and actual mass.
b
Mass pairs and the corresponding cross-linked sites in bold were confirmed by MS/MS.