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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Innate Immun. 2013 May 31;20(1):10.1177/1753425913485308. doi: 10.1177/1753425913485308

Figure 1.

Figure 1

Incorporation of [14C]acetate into fatty acids of Francisella and E. coli. (A) Schematic diagram of a predominant species of Francisella lipid A. The ester- and amide-linked 3-OH-FA are indicated.12 (B) Schematic overview of the extraction and analysis of metabolically-labeled [14C]FA. (C) NP-TLC of recovered chloroform phase after lipid extraction of the indicated metabolically-labeled bacteria. Separated [14C] 3-OH-FA and NFA were eluted and further resolved by RP-TLC. Migration of 3-OH-FA and NFA standards are indicated on the left and right of the RP-TLC, respectively. Ftn, F. novicida. NFA, non-hydroxylated fatty acids. LOS, lipooligosaccharide from N. meningitidis.