Figure 6. Joint probability functions for FRET distances in common coordinate frame from two FRET pairs.
(a) Joint probability (blue) of FRET pairs from Texas red attached to the A site of the CA Scar NPF construct and GFP attached to Arp2 and ARPC3. A low-resolution representation of Arp2/3 complex (gray surface) is shown for reference. Four orthogonal views are shown, the length of the scale bar is 5 nm. (b) Joint probability (yellow) of FRET pairs from Texas red attached to the A site of the CA Scar NPF construct and GFP attached to Arp2 and ARPC1. (c) Joint probability (cyan) of FRET pairs from Texas red attached to the A site of the CA Scar NPF construct and GFP attached to ARPC1 and ARPC3.