Skip to main content
. Author manuscript; available in PMC: 2013 Oct 12.
Published in final edited form as: Cancer Prev Res (Phila). 2013 Feb 19;6(4):349–359. doi: 10.1158/1940-6207.CAPR-12-0389

Figure 3. 15-PGDH gene CpG island Promoter Methylation Status according to H pylori infection.

Figure 3

A, Gastric specimens from H pylori-positive (n=20) and -negative (n=13) subjects were subjected to methylation-specific PCR with unmethylated (U) or methylated (M) 15-PGDH primer sets. Completely unmethylated (Ctr U) or methylated (CtrM) bisulfate-converted human DNA served as a control. The human gastric cancer cell line TMK-1 served as a positive control. The white arrowheads indicate products obtained with the M-primer pair. B, The gastric specimens were analyzed by pyrosequencing. The H pylori-positive samples had a significantly higher percentage of 15-PGDH promoter DNA methylation than the H pylori-negative specimens. *indicates a statistically significant difference (P< 0.05). C, The 33 gastric specimens were divided into two groups according to whether they had >4% or ≤4% 15-PGDH promoter methylation. The >4% group had significantly less 15-PGDH mRNA expression than the ≤4% group. **indicates a statistically significant difference (P< 0.01).