Figure 3.

Mutation at a discrete set of amino acids provides resistance to silvestrol and ROC-N. Haploid yeast strains carrying a single mutated TIF1 allele in a Δ7Δtif2 background were grown in liquid culture supplemented with DMSO (left panels), ROC-N (0.7 µM, middle panels), or silvestrol (65 µM right panels). Growth was monitored by the Absorbance at 600 nM and measured over time.