Skip to main content
. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: ACS Chem Biol. 2013 May 7;8(7):1519–1527. doi: 10.1021/cb400158t

Figure 4.

Figure 4

Effect of TIF mutations and rocaglamides on RNA binding activity. A. SDS-PAGE analysis of purified recombinant TIF1 proteins. One microgram of each wild-type and mutant proteins were separated by SDS-PAGE and stained with Coomassie blue. B. RNA binding activity of TIF1 mutants. Asterisk denotes RNA control in the absence of protein. Assays were performed as described in the Methods in the presence of 0.5 %DMSO, 10 µM ROC-N, or 50 µM silvestrol (concentrations chosen based on the ability to block translation (Figure 1C and data not shown). n=3 ± SEM.

HHS Vulnerability Disclosure