Table 1.
Mutations in TIF1 enable resistance to ROC-N and silvestrol. IC50 values for individual strains were calculated at the peak of the logarithmic growth phase.
| Yeast Residue |
Resistant amino acid |
ROC-N IC50 (µM) |
Silvestrol IC50 (µM) |
|---|---|---|---|
| Thr146 | Ala | 0.35 | 65.33 |
| Pro147 | Gln | 0.40 | >200 |
| Pro147 | Ser | 0.42 | 68.20 |
| Pro147 | Leu | 0.53 | >200 |
| Phe151 | Ser | 1.03 | >200 |
| Phe151 | Leu | 0.87 | >200 |
| Phe180 | Ser | 0.68 | >200 |
| Gln183 | His | 0.73 | >200 |
| Gln183 | Pro | 0.20 | 38.83 |
| Gln183 | Glu | 0.72 | >200 |
| Gln183 | Arg | 0.48 | >200 |
| Iso187 | Met | 13.20 | >200 |
| Δ7Δtif2 | 0.18 | 34.64 | |
| Δ7 | 0.25 | 55.53 |