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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Mol Neurobiol. 2013 May 25;48(3):10.1007/s12035-013-8470-2. doi: 10.1007/s12035-013-8470-2

Figure 2.

Figure 2

Regulation of crystallin mRNA and protein expression in ocular hypertension rat model. mRNA levels of alpha and beta crystallin genes 2 (blue solid bars) and 5 (red solid bars) weeks after IOP elevation. mRNA levels of the six cristallin genes, expression of which was affected by IOP elevation as detected by gene profiling, was analyzed. Consistent with the gene profiling data, transcription of alpha A, alpha B, beta A1/A3, beta A2, beta A4 and beta B2 was transiently downregulated at 2 weeks but was similar to that of control at 5 weeks after IOP increase. Protein levels of alpha A, alpha B, beta A1/A3 and beta B2 were lower at 2 weeks (blue hatched bars) and at 5 weeks (red hatched bars) after IOP elevation compared to controls. Beta A2 and beta A4 crystallin proteins were undetected in the retinal extract, most likely due to their low expression level.