Representative in-vivo fluorescence images tracking the same subjects at 15 min (a,e), 18 h (b,f), 45h (c,g) after oral gavage and the intestines after dissection at 48h (d,h). Mice (n= 3) were administered 15 mg InvaCuInSexS2-x microparticles conjugated with (e-h) wild type invasin or (a-d) the non-binding D911A variant.