Figure 4.

Time course of ERK1/ERK2 activation and RAGE expression during repetitive cerulein administration. The phosphorylation of ERK1/ERK2 was analyzed by Western blots (arrowheads, A) and compared to ERK1/ERK2 expression (arrows, B) at the indicated time points after the first cerulein administration. The phosphorylation intensity of ERK1/ERK2 reveals a maximum of phosphorylation at 5.5 hours after the first cerulein administration (C). No obvious effect on the expression level of any of the six RAGE proteins was observed 5.5 hours after the first cerulein administration (D) compared to β-tubulin expression (E).