Table 1.
Mass spectrum analysis results
| Protein description | Molecular function | Pep count | Unique Pep count |
|---|---|---|---|
| Yki mass spectrum | |||
| Brahma (Brm) | ATP-dependent helicase | 11 | 5 |
| Osa | DNA binding | 5 | 4 |
| Sd mass spectrum | |||
| Brahma associated protein 55kD (Bap55) | Structural constituent of cytoskeleton | 8 | 5 |
| Brahma associated protein 60kD (Bap60) | Protein binding | 5 | 3 |
| Brahma (Brm) | ATP-dependent helicase | 4 | 4 |
| Brahma associated protein 155 kDa (Mor) | Protein binding | 1 | 1 |
To determine whether there are physical interactions between Yki/Sd transcriptional complex and Brm complex and gain further understanding of the regulation mechanism of Brm in regulating ISC proliferation, we immunoprecipitated endogenous Sd or Yki protein in S2 cells using generated rabbit anti-Sd or anti-Yki antibodies, respectively, followed by mass spectrometry (MS) analysis. The corresponding proteins of Brm complex identified in association with Yki (Yki mass spectrum) or Sd (Sd mass spectrum) are listed with the number of peptides identified by mass spectrometry.