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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol. 2013 Aug 23;191(7):3884–3895. doi: 10.4049/jimmunol.1301344

Figure 10. Increased BAL and serum IL-18 and IL-1β levels in IFrag−/− mice are associated with increased induction of inflammasome-mediated Caspase-1 activity in cellular sources of BAL and lung digest.

Figure 10

Caspase-1 activity was evaluated on a single cell level in cellular sources of BAL fluid and lung digest of IFrag−/− and RAG−/− mice at day 0, 7, 10 and 16 of Pneumocystis lung infection using a live cell assay in which fluorescently-labeled Caspase-1 inhibitor (FAM-Flicka) enters the cell and binds irreversibly to activated caspase-1, allowing subsequent FACS analysis in combination with cell surface marker staining. Figure 10 A shows the percentage of active Caspase-1+ cells in BAL fluid and Figure 10B shows the percentage of caspase-1+ cells in lung digest single cell suspensions. Statistical analysis was performed using a 2-way ANOVA. Comparisons were made between groups at each time point. P values are marked as * p< 0.05, ** p<0.01, *** p<0.001.