Comparative serum cytokine analysis was performed on IFrag−/− and RAG−/− mice at day 0, 7 10 and 16 post Pneumocystis lung infection using a multiplex ELISA-based assay to evaluate if other pro-inflammatory and IFNγ-regulatory cytokines are uniquely induced in IFrag−/− mice. Shown are comparative data for IFNγ (A), IL-12p70 (B), IL-18 (C), IL-1β (D), IL-15 (E) and IL-10 (F). Statistical analysis was performed using a 2-way ANOVA. Comparisons reflect differences between the groups at each individual time point. P values are marked as * p< 0.05, ** p<0.01, *** p<0.001.