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Example of HER2 gene mutation analysis. The double peaks on path 3 refer to two PCR products, differing in base pair number: The shorter, consisting of 342 base pairs, is typical for the HER2 wild-type (WT) gene and the longer (insertion of 12 base pairs), is characteristic for mutated (MT) HER2. The single peak, containing 342 base pairs on paths 7–9 indicates the presence of only WT HER2 genes.
