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Effects of miR-99 on HeLa cell growth. (A) miR-99/5s rRNA ratio. qPCR showed that miR-99 was expressed effectively in HeLa cells following pU6.1/miR-99 transfection. (B) GFP-positive cells were used to reflect the plasmid transfection rate, which is shown to be >75%. (C) The number of living HeLa cells was lower in pU6.1/miR-99-treated cells compared with that in control plasmid-treated (pRNAi-U6.1/Neo) cultures. rRNA, ribosomal RNA; GFP, green fluorescent protein.
