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Cell proliferation and mortality were analyzed using MTT and Trypan blue assays. (A) HeLa cell growth was suppressed significantly by miR-99 following pU6.1/miR-99 transfection compared with that of the controls (P<0.01). (B) The Trypan blue results showed that there was a higher mortality rate in pU6.1/miR-99-treated cells compared with that of the controls (P<0.01). MTT, 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
