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. 2013 Oct 14;8(10):e77206. doi: 10.1371/journal.pone.0077206

Figure 4. Sensitivity of SSR systems to NHEJ and DNA send resection impairment.

Figure 4

Effects of shRNA-mediated depletion of core NHEJ or DNA end resection factors for the SSR 1.0 (A) and SSR 2.0 (B). To calculate the deviation from the balance between homology-dependent versus homology-independent repair, the ratio between green versus red cells in each conditions was calculated. To facilitate comparing experiments, this ratio was normalized for each shRNA with a scrambled sequence shRNA as a control. Those shRNAs that skewed the balance towards an increase in homology-independent repair have a fold-increase of over 1 (green bars), while those with an increase in HR have a fold-decrease of less than one (red bars). Data represent a minimum of three sets of duplicated experiments.