Figure 3. IL-10 mRNA responses to UPEC in dual co-cultures that contain membrane inserts are principally monocyte-derived.

(A) Monocytes in dual co-cultures overall have > 10-fold abundance of IL-10 transcript compared to uroepithelial cells, as transcripts emerge 3.4 cycles (Ct = 25.0 vs Ct = 28.4) earlier in qPCR (monocyte vs uroepithelial cell Ct values, p = 0.007). (B) Monocytes from all insert-containing infected conditions showed a statistically significant increase in IL-10 mRNA (1.8-fold increase of averaged infected monocyte conditions over control non-infected monocytes; insert-containing infected monocytes from dual co-cultures vs uninfected control, * p = 0.009). Uroepithelial cells from insert-containing infected conditions did not show significant increases over non-infected (1.16-fold increase of averaged infected uroepithelial cell conditions over control non-infected uroepithelial cells). Mann–Whitney U-tests were used for comparisons and SEM bars are shown.