Figure 5. Immunofluorescent images showing the absence of Xbp1 in cells expressing Zhangfei but not Zhangfei with a mutated leucine zipper.

Vero cells were transfected with plasmids expressing either Xbp1 alone or in combination with either Zhangfei or ZF(L/A). Cells were then fixed and incubated with a mixture of rabbit anti-Zhangfei and mouse anti-Xbp1 antibodies. Antibodies were visualized by staining with Alexa546 (red) anti mouse and Alexa488 (green) anti-rabbit antibodies.