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. 2013 Oct 14;8(10):e77322. doi: 10.1371/journal.pone.0077322

Figure 2. Small portion of human CD4+ memory T cells can be converted to Foxp3+ T cells in vitro.

Figure 2

(A) CD4+ naive (Tn) and memory (Tm) T cells were sorted and activated with plate-bound anti-CD3 (5 μg/ml) and soluble anti-CD28 (1 μg/ml) in the presence or absence of TGF-β (5 ng/ml) and IL-2 (100 UI/ml). Foxp3 expression was analysed by flow cytometry at different time points as indicated. (B) The average percentages of Foxp3 expression in CD4+ naive and memory T cells on day 5 after TGF-β and IL-2 induction. (C) and (E) Flow cytometric analysis of CTLA-4 (extracellular and total), PD-1, and PD-L1 expression in total, freshly sorted, naive (Tn) or memory (Tm) CD4+ T cells. (D) and (F) Sorted CD4+ naive (Tn) or memory (Tm) T cells were activated for 5 days as described above, and the total population was analysed using flow cytometry for the expression of CTLA-4 (extracellular and total), PD-1, and PD-L1.