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. 2013 Oct 14;8(10):e77293. doi: 10.1371/journal.pone.0077293

Figure 2. siRNA/HOTAIR significantly increases the in vitro sensitivity of A549/DDP to cisplatin.

Figure 2

(A) 48h after A549/DDP cells transfected with siRNA/control, siRNA/HOTAIR1 or siRNA/HOTAIR2, qRT-PCR detection of HOTAIR expression in those cells. GAPDH was used as an internal control. (B) MTT analysis of the IC50 values of cisplatin to siRNA/HOTAIR1 or siRNA/control-transfected A549/DDP cells. (C) Flow cytometry analysis of apoptosis in siRNA/control or siRNA/HOTAIR1-transfected A549/DDP cells combined with various concentrations of cisplatin (0.0, 1.0 or 2.0 μg/L). (D) Flow cytometry analysis of cell cycle distribution in siRNA/control or siRNA/HOTAIR1-transfected A549/DDP cells combined with various concentrations of cisplatin (0.0, 1.0 or 2.0 μg/L). Results represent the average of three independent experiments (mean±SD). N.S indicates P>0.05 and * or ** indicates P<0.05 or <0.01, respectively.