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. 2013 Oct 14;8(10):e76170. doi: 10.1371/journal.pone.0076170

Figure 3. Transcriptional analysis of agu genes in E. faecalis.

Figure 3

A) Schematic representation of agu operons. PaguR and PaguB indicate promoter regions. Secondary structures TaguR and TaguB represent putative Rho-independent transcriptional terminators. B) Northern blot analysis. E. faecalis wild-type (lanes 1 and 2) or aguR (lane 3) cells were grown in LB Gal with or without agmatine (Ag). Total RNA (extracted after 6 h of growth) was hybridized against specific probes I or II. Transcript size was determined by comparison to RNA markers. C) Primer extension experiments for the determination of aguR and aguB transcriptional start sites (lanes +1). Lanes G, A, C and T show homologous sequence ladders. D) Nucleotide sequence of aguR-aguBDAC intergenic region. Positions of transcriptional start sites are indicated (+1). Putative Shine-Dalgarno (SD), −10 and −35 regions are shown underlined. Translational start sites are shown in bold. The predicted cre site is highlighted in purple.